LentiGlo ^TM  PDF booklet

General information about Lentiviral vectors (LV) are vehicles that can deliver genes or RNAi into cells with up to 100% efficiency and stability. By comparison, other viral vector systems such as non-viral, adenoviral and adeno-associated viral vectors have not been shown to achieve both

high and stable gene delivery in dividing cells.

Gene delivery is accomplished by the binding and fusing of the LV pseudotyped envelope protein to the target cell membrane. The LV RNA containing the gene or gene silencing sequence is then incorporated into the cell via reverse transcription creating a

DNA complex. This complex enters the nucleus incorporating into the chromosomal DNA creating a stable molecule. The gene sequence is integrated in the chromosome and is copied along with the DNA during ongoing cell division.

The LentiGlo ^TM  lentiviral vectors are lentiviruses express different bioluminescent genes under control of the CMV promoter.

Three lentiviral vectors are currently available:

LentiGlo ^TM ‘Gluc: A lentiviral vector expressing gaussia luciferase under control of the CMV promoter

LentiGlo ^TM GFP:  A lentiviral vector expressing green fluorescent protein under control of the CMV promoter

LentiGlo ^TM Gluc-GFP:  A lentiviral vector expressing gaussia luciferase under control of the CMV promoter and a green fluorescent protein under an IRES.